malS [2017-8-11 16:0:3]

malate dehydrogenase (decarboxylating), forms a transhydrogenation cycle with YtsJ for balancing of NADPH

locus: BSU29880

pI: 4.89

mw: 61.98 kDa

protein length: 566 aa

gene length: 1698 bp

function: malate utilization

product: malate dehydrogenase (decarboxylating)

essential: no

ec: 1.1.1.38

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]

Gene

Coordinates: 3,056,849 → 3,058,549

The protein

Catalyzed reaction/ biological activity

• (S)-malate NAD = pyruvate CO₂ NADH (according to Swiss-Prot) malate -→ pyruvate

Protein family

• malic enzymes family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|4F30A4412768E6313F57BD56C4EF5079F500E0C4|MaeA] [protein|45229A538D5124B60CE05DDC8699B9BC3D5AD9FF|YtsJ] [protein|160EBB7885D7C7FD30A6E35605A862C156E2DA80|MleA]

Structure

• [PDB|1LLQ] (from ''Ascaris suum'', 40% identity) [Pubmed|12033925]

Expression and Regulation

Operons

• genes: [gene|53259B300509E6B6DAF3ED106D1DDEDC99B5532B|ytnP]-[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]
  description: [Pubmed|22383849]

• genes: [gene|BBBCD5F56779895189E21076AF165B901F654534|malS]
  description: [Pubmed|9387221]

Biological materials

Mutant

• GP1142 (Δ[gene|BBBCD5F56779895189E21076AF165B901F654534|malS]::spc) available in [SW|Jörg Stülke]'s lab

References

• 9387221,16788182,12949160,23136871,22740702,12033925